Skip to main content
. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: Bioconjug Chem. 2011 Sep 29;22(10):2143–2153. doi: 10.1021/bc200415v

Figure 2.

Figure 2

Potential mechanisms for the selection of hydrazide interacting peptides from phage-displayed libraries include oxidation of susceptible peptides (1) for capture with hydrazide-derivatized Tentagel via hydrazone bond formation. Additionally, phage-displayed peptides could form high affinity non-covalent interactions with the hydrazide functionality (2). Alternatively, the hydrazide functional group could be oxidized and attacked by a peptide nucleophile liberating nitrogen gas (3). Lastly, a nucleophile from the displayed peptide could attack the carbonyl of the hydrazide functional group, displacing hydrazine (4).