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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cytometry A. 2011 Jul 27;79(11):920–932. doi: 10.1002/cyto.a.21111

Figure 1. UV absorption images and mass maps.

Figure 1

UV images at (A) 280 nm, (B) 260 nm, (C) 235 nm, (D) 220 nm, and (E) 200 nm. Pixel values are expressed as optical density (OD) values, calculated as the logarithm of the ratio of the background and cell images. Images of the calculated nucleic acid (F) and protein mass (G), based on 260-nm and 280-nm UV images. The mass maps (F,G) are normalized to a maximum of 1.1×10−14 and 2.0×10−13 pg per 0.077-μm2 pixel (140 fg and 2.6 pg per square micrometer peak nucleic acid and protein density, respectively). NA: nucleic acid.