Table 1.

Molar extinction coefficients by UV wavelength

Wavelength (nm)	nucleic acid (M−1 cm−1)	protein (M−1 cm−1)
280	3500#	54129#
260	7000#	36057#
220	4000#*	432500*,**,346000##
200	10500#*	768000*,**

^#Taken from (1), values for hybridized averaged nucleic acid.

^#*Taken from (32)

^*calculated using formula from (27)

^**Table 1 provides the calculated value of the molar extinction coefficient for average protein in a random coil conformation.

^##The molar extinction coefficient at 220-nm was calibrated empirically; see Figure 3.