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. Author manuscript; available in PMC: 2012 Oct 21.
Published in final edited form as: ACS Chem Biol. 2011 Aug 10;6(10):1015–1020. doi: 10.1021/cb200164h

Figure 3. Effect of C-terminal amino acids on CPY-catalyzed protein biotinylation.

Figure 3

(A) GST fusion proteins containing each of twenty amino acids at the C-terminus can be biotinylated with CPY. Each GST protein (1 µg) was methyl esterified and incubated with CPY and biocytinamide at 37°C for 30 min. Because of the limited number of samples that can be loaded per gel, these experiments were conducted on two blots that were Western blotted with an anti-biotin antibody and exposed simultaneously.

(B) CPY-mediated biotinylation of naturally occurring proteins. The indicated proteins (1 µg) were methyl esterified and labeled with CPY and biocytinamide. The top band in the myoglobin lane is a contaminant.