Table 2.
Reagents used for OMIP-000.
| Specificity | Clone | Fluorochrome | Purpose |
|---|---|---|---|
| IgD | IA6-2 | FITC | Differentiation |
| CXCR3 | 1C6/CXCR3 | PE | Homing |
| CD24 | SN3 | PE-Alexa610 | Differentiation, epithelial adhesion |
| CD21 | B-ly4 | PE-Cy5 | Activation, BCR co- receptor for antigen- bound C3d |
| CD38 | HIT2 | PerCP-Cy5.5 | Differentiation |
| CD45/B220 | RA3/6B2 | PE-Cy7 | Differentiation |
| CD3 | SP-34-2 | Pacific Blue | Exclusion |
| Dead cells | Aqua (Pacific Orange) | Exclusion | |
| CD27 | CLB-27/1 | Qdot605 | Differentiation, receptor for CD70 on activated T cells |
| CD95 | DX2 | APC | Activation; pro- apoptotic in the presence of activated CD95L T cells |
| VH4-34-encoded idiotype | 9G4 | Biotin | Autoreactive B cells |
| Biotin | Streptavidin | Alexa680 | |
| CD19 | SJ25C1 | APC-Cy7 | Lineage |
FITC, fluorescein isothiocyanate; PE, R-phycoerythrin; Cy, cyanine; PerCP, peridinin-chlorophyll protein; Aqua, LIVE/DEAD fixable aqua dead cell stain; Qdot, quantum dot; APC, allophycocyanin.