Figure 1.
TSP-1 upregulation in the pressure-overloaded heart. A. Quantitative rt-PCR demonstrated that TSP-1 mRNA was markedly induced in the pressure overloaded myocardium after 3-7 days of TAC (*p<0.01 vs. sham; *p<0.05 vs. sham). B-C: Immunohistochemical localization of TSP-1 protein in the myocardium after 7 (B) and 28 days (C) of TAC. TSP-1 was predominantly localized in the perivascular and interstitial space (arrows). Sections were counterstained with eosin. D. Kaplan-Meier curves demonstrating survival rates of WT and TSP-1 null mice undergoing pressure overload protocols. TSP-1 null mice and WT animals showed comparable mortality after 28 days of TAC (pNS), but had lower survival rates during the first 3 days of pressure overload (p=0.05).