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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Hypertension. 2011 Sep 6;58(5):888–894. doi: 10.1161/HYPERTENSIONAHA.110.167809

Figure 1. Generation of NCC transgenic mice.

Figure 1

(A) BAC clone RP-24-263E2 was modified by recombineering in E. coli, to remove Herpud1 and the region of Nup43 extending to its 3′ untranslated region (UTR). The resulting NCC transgene contains 12.5kb of sequence upstream of the NCC transcription start, and the 3′ UTR of NCC (vector sequence is not shown). (B) Semi-quantitative PCR on genomic DNA extracted from the tails of potential Founders identified lines 727 and 743 as containing >12 and 5 copies of the NCC transgene; amplification of the β-globin gene confirmed equal template input.