Table 2. Temperature-dependent cellular effects of holC mutations in E. coli.
Temperature | Strain | holC | pepA | Relative fluorescence intensity | % SOS induced | % Filamented |
---|---|---|---|---|---|---|
SS8343 | + | + | 1.0±0.0 | 0.4±0.6 | 0.1 | |
SS8383 | + | cat | 1.9±0.8 | 1.4±1.0 | 0.4 | |
30°C | SS8384 | R128A | cat | 1.7±0.7 | 1.9±1.2 | 0.1 |
SS8385 | R128E | cat | 1.4±0.5 | 1.0±0.9 | 1.0 | |
SS8386 | K132E | cat | 1.3±0.9 | 1.0±0.7 | 0.1 | |
SS8387 | R128E K132E | cat | 2.0±0.7 | 2.9±2.2 | 1.0 | |
SS8343 | + | + | 1.0±0.0 | 0.5±0.4 | 0.1 | |
SS8383 | + | cat | 1.0±0.3 | 0.6±0.2 | 0.2 | |
37°C | SS8384 | R128A | cat | 1.1±0.7 | 1.0±0.8 | 0.1 |
SS8385 | R128E | cat | 1.4±0.6 | 2.3±1.6 | 1.4 | |
SS8386 | K132E | cat | 1.1±0.1 | 1.0±0.7 | 0.0 | |
SS8387 | R128E K132E | cat | 2.4±0.4 | 6.5±2.3 | 4.0 | |
SS8343 | + | + | 1.0±0.0 | 1.1±0.9 | 0.2 | |
SS8383 | + | cat | 1.1±0.6 | 0.3±0.4 | 0.6 | |
42°C | SS8384 | R128A | cat | 1.1±0.3 | 0.5±0.1 | 0.4 |
SS8385 | R128E | cat | 1.9±0.6 | 4.2±1.5 | 2.4 | |
SS8386 | K132E | cat | 1.1±0.5 | 0.6±0.3 | 0.3 | |
SS8387 | R128E K132E | cat | 4.1±0.3 | 17.3±3.0 | 6.0 |