TABLE 2.

Effect of fatty acid chain length and saturation on percentage of substrate hydrolyzed by PNPLA3

TAGs and DAGs containing a single species of fatty acid (myristate, palmitate, oleate, linoleate, and arachidonate) were prepared as substrates at a concentration of 200 μm. Substrates were incubated with purified PNPLA3 at 37 °C. The acylglycerol substrates (TAG or DAG) and released free fatty acids were isolated and quantified by GC-MS. The percentages of hydrolyzed substrates were calculated. Each reaction was repeated at least three times. NA, not applicable.

Fatty acid	TAG	DAG
Myristate (14:0)	5.4 ± 1.6	NA
Palmitate (16:0)	5.7 ± 0.4	6.0 ± 2.6
Oleate (18:1)	15.4 ± 1.1	15.2 ± 3.5
Linoleate (18:2)	5.4 ± 0.2	7.2 ± 0.7
Arachidonate (20:4)	5.6 ± 1.6	NA