FIGURE 4.

tTG and Hsp70 co-localize to the leading edges of cells. A, HeLa and MDAMB231 cells ectopically expressing V5-Hsc70 and Myc-tTG were fixed and then subjected to immunofluorescence using V5 and Myc antibodies and DAPI (to stain nuclei). Representative images of the transfectants are shown, with the co-localization of the ectopically expressed forms of tTG and Hsc70 at leading edges being highlighted with arrows. B, serum-starved HeLa cells and MDAMB231 cells were treated without (Untreated) or with EGF for 12 h, as indicated, and fixed. Immunofluorescence was performed on the cells using tTG and Hsp70 antibodies and DAPI. Representative images of the cells are shown, with the co-localization of tTG and Hsp70 at leading edges being indicated with arrows.