FIGURE 5.

Inhibiting Hsp70 activity blocks the ability of tTG and Hsp70 to localize at leading edges. A, serum-deprived HeLa cells were treated without (Untreated) or with EGF, with or without myricetin, methylene blue, or VER 155008, and then immunofluorescence was performed on the cells using tTG and Hsp70 antibodies. The resulting fluorescent images are shown with the tTG and Hsp70 at leading edges being indicated with arrows. B, quantification of the cells shown in A with tTG at their leading edges. C, serum-starved MDAMB231 cells were treated without (Untreated) or with myricetin, methylene blue, or VER 155008, and then immunofluorescence was performed on the cells using tTG and Hsp70 antibodies. The resulting fluorescent images are shown with the tTG and Hsp70 at leading edges being indicated with arrows. D, quantification of the cells shown in C with tTG at their leading edges. Three independent experiments were performed, with at least 250 cells being scored for each condition. The results from the experiments were then averaged together and graphed. The error bars indicate standard deviation.