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. 2011 Sep 6;286(43):37094–37107. doi: 10.1074/jbc.M111.242438

FIGURE 6.

FIGURE 6.

Inhibition of Hsp70 does not have a global effect on leading edge proteins. A, serum-deprived HeLa cells were treated without (Untreated) or with EGF, with or without myricetin, methylene blue, or VER 155008, and then immunofluorescence was performed on the cells using a tTG antibody, rhodamine-conjugated phalloidin (Actin), and DAPI. The resulting fluorescent images are shown with the tTG at leading edges being indicated with arrows. B, serum-starved MDAMB231 cells were treated without (Untreated) or with myricetin, methylene blue, or VER 155008, and then immunofluorescence was performed on the cells using a tTG antibody, rhodamine-conjugated phalloidin (Actin), and DAPI. The resulting fluorescent images are shown with the tTG at leading edges being indicated with arrows. C, MDAMB231 cells were transiently transfected with either of two HA-tagged constructs: constitutively active Rac (F28L) or GTP hydrolysis-defective Ras (G12V), and immunofluorescence was performed using HA and tTG antibodies and DAPI. The resulting fluorescent images are shown with the Rac, Ras, and tTG at leading edges being indicated with arrows.