FIGURE 8.

Inhibition of Hsp70 blocks the migration of HeLa and MDAMB231 cells. A, scratch assays were performed on serum-deprived HeLa cells treated without (Untreated) or with EGF, with or without myricetin, and on serum-starved MDAMB231 cells with or without myricetin. The MDAMB231 cells were fixed 12 h after striking the wound; HeLa cells were fixed after 24 h. The cells were then visualized using light microscopy, and the extent of wound closure was determined. One set of untreated cells was fixed immediately after striking the wound (Untreated 0 h.) to indicate the size of the initial wounds. The widths of the initial wounds are indicated by dashed lines. B, scratch assays were performed on the cell lines using methylene blue as outlined in A. C, scratch assays were performed on the cell lines using VER 155008 as outlined in A.