FIGURE 1.

Functional repression of AR by LCoR. A, Western blot analysis showing LCoR proteins in a panel of prostate cell lines. β-actin was used as a loading control. B, CV1 cells were transfected with pMMTV-Luc, WT hAR, and increasing amounts of pSG5-LCoR (5–40 ng). Cells were transfected with 1 μg of WT hAR (C) or T877A mutant (D). E, CV1 cells were transfected with GRE-Luc, pARR3-Luc (probasin), or PSA-Luc (F) along with WT hAR and pSG5-LCoR. The graph represents the fold hormone induction with S.D. between triplicates. LCoR-mediated AR repression was significant in C–F (Student's t test, p < 0.005). LCoR-mut represents the NR-box mutant of LCoR (12). AR ligands (agonists): DHT, R1881. AR ligands (antagonists): OHF, Casodex, CPA. G, stable clones of C4-2 cells overexpressing either the empty vector control (left panel) or overexpressing LCoR (right panel) and the number of colonies obtained from the corresponding stable clones (H).