FIGURE 2.

Mutational analysis of AR-LCoR-mediated repression. A, CV1 cells were transfected with the pMMTV-Luc reporter along with the pSG5 or pSG5-LCoR expression vectors and treated with agonist R1881. LCoR-mediated AR repression was significant (Student's t test, p < 0.001). B, various AR deletion plasmids that lead to truncated AR proteins were cotransfected with LCoR and later treated with agonist R1881. LCoR-mediated AR repression was significant (Student's t test, p < 0.01). C, CV1 cells were transfected with pMMTV-Luc along with pSG5-LCoR-mut. In addition, various AR mutant expression plasmids were also transfected, and cells were treated with R1881. The graph represents the fold hormone induction. LCoR mutant-mediated AR repression was significant (Student's t test, p < 0.05).