FIGURE 3.

Interaction analysis in mammalian cells: NTD and DBD are required. A and B, CV1 cells were transfected with pMMTV-Luc, VP16, or VP-DBD-LBD (0.1 μg) and 1 μg each of either pSG5, pSG5-LCoR, or pSG5-LCoR-mut and were incubated with R1881. The graph represents fold hormone activity. C, cells were transfected with MMTV-Luc, VP16 empty, and VP16-Gal-DBD (called VP-Gal) as controls or VP-DBD (1 μg), pSG5 alone, LCoR, or LCoR-mut and treated with R1881. LCoR-mediated repression of the VP-AR deletion mutants was significant (Student's t test, p < 0.005). The graph represents the fold hormone induction.