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The relative abundance of Npt2a, Npt2c, NHERF-1, and ezrin was determined by quantitative immunoblotting in BBM vesicles from control (−) or FGF-23 (10−9m) treated wild-type kidney slices. A, representative immunoblots. B, summary of BBM vesicle abundance after hormone treatment (+) expressed relative to non-hormone-treated control (−) BBM vesicles ± S.E. (error bars). n = 4, *, p < 0.05.
