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. 2011 Aug 31;286(43):37358–37367. doi: 10.1074/jbc.M111.272401

FIGURE 5.

FIGURE 5.

mMCP-4 produces dermal-epidermal separation of the skin and cleaves BP180 in tissue and in vitro. A, neonatal mouse skin sections were incubated with culture medium alone or with mMCP-4 (1 μg/ml) at 37 °C for 12 h and were examined by H&E staining. Dermal-epidermal separation was seen in the skin incubated with mMCP-4 (right panel) but not the medium control (left panel). E, epidermis; D, dermis. Arrow, basal keratinocytes. 100× magnification. B, protein extracts from mouse skin incubated with culture medium alone or mMCP-4 above were analyzed by immunoblotting using an anti-mBP180 antibody. mMCP-4 (lane 2), but not culture medium (lane 1), degraded BP180. C, recombinant mBP180ABC was incubated with mMCP-4 at 37 °C for 0–60 min. The digestion products were resolved by SDS-PAGE and detected by immunoblotting using an anti-mBP180 antibody. Degradation products were seen after incubation with mMCP-4 for 5, 15, 30, and 60 min.