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. 2011 Sep 9;286(43):37414–37428. doi: 10.1074/jbc.M111.287649

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — OX₁ receptor antagonists block effects of orexin A at both OX₁ and CB₁ receptors. Clone B6 was untreated (open symbols) or treated with doxycycline (10 ng·ml⁻¹ for 24 h) (filled symbols) to induce expression of VSV-G-SNAP-CB₁. Cell surface VSV-G-SNAP-CB₁ (A and B) and HA-CLIP-OX₁ (C and D) were measured, respectively, by the binding of SNAP-Lumi4Tb (A and B) and CLIP-Lumi4Tb (C and D) after treatment of cells for 40 min with 5 × 10⁻⁷ m orexin A and varying concentrations of the OX₁ receptor antagonists SB408124 (A and C) and SB334867 (B and D). Data are means ± S.E. (n = 3). VS, VSV-G + SNAP tag; HC, HA + CLIP tag.