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. 2011 Sep 9;286(43):37414–37428. doi: 10.1074/jbc.M111.287649

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Orexin A-induced CB₁ receptor internalization is blocked by CB₁ receptor antagonists. Clone B6 (A and C) or clone C2 (B) cells were induced to express the harbored receptor (VSV-G-SNAP-CB₁ (A and C) or HA-CLIP-OX₁ (B)). The effects of varying concentrations of the CB₁ antagonists O-2050 (A and B) and AM251 (C) to modulate internalization of the CB₁ receptor mediated by treatment with 1 × 10⁻⁶ m orexin A for 40 min were assessed by the binding of SNAP-Lumi4Tb. Data are means ± S.E. (n = 3). VS, VSV-G + SNAP tag; HC, HA + CLIP tag.