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. 2011 Aug 30;286(43):37429–37445. doi: 10.1074/jbc.M111.271494

FIGURE 2.

FIGURE 2.

Air1 zinc knuckle 4-5 linker IWRXY motif and zinc knuckle 5 residues are functionally important. air1 IWRXY motif mutants (air1-I152A, -W153A, -R154A, and -Y156A) and air1 ZnK5 mutants (air1-Y176A, -F184A, and -D186A) exhibit temperature-sensitive growth. air1Δ air2Δ cells containing AIR2 URA3 maintenance plasmid and vector, AIR1, air1 IWRXY mutant (air1-I152A, -W153A, -R154A, or Y156A), air1 ZnK5 mutant (air1-F174A, -Y176A, -F184A, -D186A, or -R192A), or air1 ZnK4 and -5 mutant (air1-C139R or -C178R) LEU2 test plasmids were grown to saturation, serially diluted, and spotted on control and 5-FOA plates at the indicated temperatures.