FIGURE 3.

Air1 zinc knuckles 1–5 are important for the degradation of NEL025c CUT RNA. air1 ZnK1–5 mutants (air1-C79R, -C97R, -C117R, -C139R, and -C178R) show elevated levels of NEL025c CUT RNA relative to wild type cells. Total RNA from wild type, air2Δ, trf4Δ, rrp6Δ, and air1Δ air2Δ cells containing only AIR1 or air1 ZnK1–5 mutant plasmid grown at 25 °C was measured by quantitative RT-PCR using gene-specific primers for the CUT NEL025c as described under “Experimental Procedures.” Relative NEL025c RNA levels were measured in triplicate biological samples, normalized to a control PGK1 transcript by the ΔΔCt method, averaged, and graphically shown as -fold increase relative to wild type cells. Error bars, S.E.