TABLE 1.
Yeast strains and plasmids
| Strain/Plasmid | Description | Source |
|---|---|---|
| Strain | ||
| W303 wild type (ACY233) | MATα ura3Δ leu2Δ trp1Δ his3Δ | |
| BY4741 wild type (ACY402) | MATa leu2Δ ura3Δ his3Δ TRP1 met15Δ | Research Genetics |
| air1Δ (ACY1090) | MATa leu2Δ ura3Δ his3Δ TRP1 AIR1::KAN | Research Genetics |
| air2Δ (ACY1091) | MATa leu2Δ ura3Δ his3Δ TRP1 AIR2::KAN | Research Genetics |
| rrp6Δ (ACY1641) | MATa leu2Δ ura3Δ his3Δ TRP1 RRP6::KAN | Research Genetics |
| trf4Δ (ACY2149) | MATa leu2Δ ura3Δ his3Δ TRP1 TRF4::KAN | Research Genetics |
| air1Δ air2Δ (ACY1095) | MATa leu2Δ ura3Δ his3Δ TRP1 met15Δ AIR1::KAN AIR2::KAN (pAC1614) | This study |
| air1-C178R air2Δ (ACY2020) | MATα ura3Δ leu2Δ trp1Δ his3Δ air1-C178R AIR2::NAT | This study |
| air1Δ air2ΔTRF4-TAP (ACY2131) | ura3Δ leu2Δ trp1Δ his3Δ AIR1::NAT AIR2::HPH TRF4-TAP:Sphis5+ | This study |
| AIR1-TAP (ACY1061) | MATa ura3Δ leu2Δ his3Δ met15Δ TRP1 AIR1-TAP:Sphis5+ | Open Biosystems |
| Plasmid | ||
| pRS416 | CEN, URA3, AMPR | Ref. 65 |
| pAC1613 | AIR1, CEN, URA3, AMPR | This study |
| pAC1614 | AIR2, CEN, URA3, AMPR | This study |
| pRS415 | CEN, LEU2, AMPR | Ref. 65 |
| pAC1856 | AIR1, CEN, LEU2, AMPR | This study |
| pAC2727 | air1-C79R, CEN, LEU2, AMPR | This study |
| pAC2728 | air1-C97R, CEN, LEU2, AMPR | This study |
| pAC2729 | air1-C117R, CEN, LEU2, AMPR | This study |
| pAC2730 | air1-C139R, CEN, LEU2, AMPR | This study |
| pAC2012 | air1-C178R, CEN, URA3, AMPR | This study |
| pAC2845 | air1-I152A, CEN, LEU2, AMPR | This study |
| pAC2837 | air1-W153A, CEN, LEU2, AMPR | This study |
| pAC2884 | air1-R154A, CEN, LEU2, AMPR | This study |
| pAC2846 | air1-Y156A, CEN, LEU2, AMPR | This study |
| pAC2847 | air1-F174A, CEN, LEU2, AMPR | This study |
| pAC2838 | air1-Y176A, CEN, LEU2, AMPR | This study |
| pAC2848 | air1-F184A, CEN, LEU2, AMPR | This study |
| pAC2839 | air1-D186A, CEN, LEU2, AMPR | This study |
| pAC2840 | air1-R192A, CEN, LEU2, AMPR | This study |
| pAC2825 | AIR1-GFP, CEN, LEU2, AMPR | This study |
| pAC2826 | air1-C79R-GFP, CEN, LEU2, AMPR | This study |
| pAC2827 | air1-C97R-GFP, CEN, LEU2, AMPR | This study |
| pAC2828 | air1-C117R-GFP, CEN, LEU2, AMPR | This study |
| pAC2829 | air1-C139R-GFP, CEN, LEU2, AMPR | This study |
| pAC2830 | air1-C178R-GFP, CEN, URA3, AMPR | This study |
| pAC2863 | air1-I152A-GFP, CEN, LEU2, AMPR | This study |
| pAC2841 | air1-W153A-GFP, CEN, LEU2, AMPR | This study |
| pAC2944 | air1-R154A-GFP, CEN, LEU2, AMPR | This study |
| pAC2864 | air1-Y156A-GFP, CEN, LEU2, AMPR | This study |
| pAC2865 | air1-F174A-GFP, CEN, LEU2, AMPR | This study |
| pAC2842 | air1-Y176A-GFP, CEN, LEU2, AMPR | This study |
| pAC2866 | air1-F184A-GFP, CEN, LEU2, AMPR | This study |
| pAC2843 | air1-D186A-GFP, CEN, LEU2, AMPR | This study |
| pAC2844 | air1-R192A-GFP, CEN, LEU2, AMPR | This study |
| pAC2885 | AIR1–2xMyc, CEN, LEU2, AMPR | This study |
| pAC2886 | air1-C79R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2887 | air1-C97R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2888 | air1-C117R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2889 | air1-C139R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2890 | air1-C178R-2xMyc, CEN, URA3, AMPR | This study |
| pAC2906 | air1-I152A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2902 | air1-W153A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2937 | air1-R154A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2907 | air1-Y156A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2908 | air1-F174A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2903 | air1-Y176A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2909 | air1-F184A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2904 | air1-D186A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2905 | air1-R192A-2xMyc, CEN, LEU2, AMPR | This study |
| pAC1857 | AIR2, CEN, LEU2, AMPR | This study |
| pAC2731 | air2-C66R, CEN, LEU2, AMPR | This study |
| pAC2732 | air2-C84R, CEN, LEU2, AMPR | This study |
| pAC2733 | air2-C104R, CEN, LEU2, AMPR | This study |
| pAC2734 | air2-C126R, CEN, LEU2, AMPR | This study |
| pAC2013 | air2-C167R, CEN, LEU2, AMPR | This study |
| pAC2831 | AIR2-GFP, CEN, LEU2, AMPR | This study |
| pAC2832 | air2-C66R-GFP, CEN, LEU2, AMPR | This study |
| pAC2833 | air2-C84R-GFP, CEN, LEU2, AMPR | This study |
| pAC2834 | air2-C104R-GFP, CEN, LEU2, AMPR | This study |
| pAC2835 | air2-C126R-GFP, CEN, LEU2, AMPR | This study |
| pAC2836 | air2-C167R-GFP, CEN, LEU2, AMPR | This study |
| pAC2891 | AIR2–2xMyc, CEN, LEU2, AMPR | This study |
| pAC2892 | air2-C66R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2893 | air2-C84R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2894 | air2-C104R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2895 | air2-C126R-2xMyc, CEN, LEU2, AMPR | This study |
| pAC2896 | air2-C167R-2xMyc, CEN, LEU2, AMPR | This study |
| pRS426 | 2μ, URA3, AMPR | Ref. 66 |
| pCB727 | TRF4, 2μ, TRP1, AMPR | M. F. Christman |
| pCB557 | TRF5, 2μ, TRP1, AMPR | M. F. Christman |
| pAC2147 | TRF4, 2μ, URA3, AMPR | This study |
| pAC2710 | trf4-DADA, 2μ, URA3, AMPR | This study |
| pAC3048 | trf4–378, 2μ, URA3, AMPR | This study |
| pAC2931 | TRF5, 2μ, URA3, AMPR | This study |
| pAC2897 | MTR4, 2μ, URA3, AMPR | This study |
| pAC2910 | TRF4–2xMyc, 2μ, URA3, AMPR | This study |
| pAC2914 | trf4-DADA-2xMyc, 2μ, URA3, AMPR | This study |
| pAC3049 | trf4–378-2xMyc, 2μ, URA3, AMPR | This study |
| pAC3050 | TRF5–2xMyc, 2μ, URA3, AMPR | This study |
| pAC3051 | MTR4–2xMyc, 2μ, URA3, AMPR | This study |
| pRS423 | 2μ, HIS3, AMPR | Ref. 66 |
| pAC2940 | TRF4, 2μ, HIS3, AMPR | This study |
| pAC2946 | trf4-DADA, 2μ, HIS3, AMPR | This study |
| pAC2998 | pcDNA3-mPAPD5–2xMyc, AMPR | This study |
| pAC2999 | pcDNA3-hPAPD7–2xMyc, AMPR | This study |