FIGURE 2.

Metabolic characteristics of adipose tissue-specific KLF15 transgenic mice. A, time course of body mass for WT and aP2-KLF15 Tg (1Tg and 3Tg) mice fed normal chow (NC) or a high fat diet (HFD). Data are means ± S.E. for 15 mice. *, p < 0.05; **, p < 0.01. B, glucose clearance during an insulin tolerance test in 17-week-old WT and aP2-KLF15 Tg mice fed a high fat diet. Plasma glucose concentration is expressed relative to the initial value. Data are means ± S.E. for 8–12 mice. *, p < 0.05. C, insulin-induced tyrosine phosphorylation of IRS-1 and Ser⁴⁷³ phosphorylation of Akt in the liver and skeletal muscle of 17-week-old WT and aP2-KLF15 Tg mice fed a high fat diet. Immunoprecipitates prepared with antibodies to IRS-1 or to Akt were subjected to immunoblot analysis with antibodies to phosphotyrosine and to IRS-1 or with those to Ser⁴⁷³-phosphorylated or total forms of Akt, respectively. D, hepatic triglyceride contents in 23-week-old WT and aP2-KLF15 Tg mice fed a high fat diet. Data are means ± S.E. for 8 mice. *, p < 0.01. E and F, plasma glucose (E) and plasma insulin (F) concentrations during an intraperitoneal glucose tolerance test in 21-week-old WT and aP2-KLF15 Tg mice fed a high fat diet. Data are means ± S.E. for 8–12 mice. *, p < 0.05; **, p < 0.01. Data in E are representative of at least three independent experiments.