FIGURE 7.

Glucose metabolism and oxidative stress in double Tg mice. A and B, plasma glucose (A) and plasma insulin (B) concentrations during an intraperitoneal glucose tolerance test in 21-week-old WT, aP2-KLF15 Tg (3Tg), SCD1 Tg, and double Tg mice maintained on a high fat diet (HFD). Data are means ± S.E. for 8 to 12 mice. *, p < 0.05; **, p < 0.01; NS, not significant. C, quantitation of β cell area as a percentage of total pancreatic area in 23-week-old mice of the indicated genotypes fed a high fat diet. Data are means ± S.E. for five mice. **, p < 0.01. D, plasma and tissue levels of lipid peroxidation evaluated from the formation of TBARS in 23-week-old mice of the indicated genotypes fed a high fat diet. Data are means ± S.E. for five mice. **, p < 0.01. E, RT and real time PCR analysis of mRNAs for the indicated proteins in WAT and BAT of 23-week-old mice of the indicated genotypes fed a high fat diet. Data are normalized by the abundance of 18 S rRNA, are expressed relative to the corresponding value for WT mice, and are means ± S.E. for five mice. **, p < 0.01. ACO, acyl-CoA oxidase.