FIGURE 8.

MLK3 modulates β-catenin pathway in prostate cancer cells. LNCaP (A), 22Rv1 (B)m and PC3 (C) were transfected with various expression vectors followed by Western blot analysis with the indicated antibodies. D and E, 22Rv1 cells were transiently transfected with pGL3OT luciferase reporter and β-galactosidase vector. In D they were co-transfected with empty vector (lane 1) or β-catenin wild-type alone (lane 2) or with increasing concentration of MLK3-WT (lanes 3 and 4). In E a stabilized mutant form of β-catenin (S37A) was co-transfected either alone (lane 2) or with increasing concentration of MLK3-WT (lanes 3 and 4). Luciferase and β-gal assays were performed as in Fig. 6A. F, 22Rv1 cells were transfected with the indicated plasmids, and after a recovery in growth medium, they were harvested and analyzed by Western blots.