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. 2011 Aug 26;286(43):37515–37524. doi: 10.1074/jbc.M111.287474

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — The composition of Idua^−/− HS is markedly different from the wild type, with significant increases in all sulfation modifications. RP-HPLC traces of AMAC-labeled heparinase-generated disaccharides from wild-type and Idua^−/− liver (a) and brain (b). Peaks are labeled following comparison to known standards: 1, HexA(2S)-GlcNS(6S); 2, HexA-GlcNS(6S); 3, HexA(2S)-GlcNS; 4, HexA-GlcNS; 5, HexA-GlcNAc(6S); 6, HexA-GlcNAc; *, minor free AMAC peak; U, uncharacterized. c and d, relative amount of HS in Idua^−/− liver (c) and brain (d) versus the wild type. Disaccharide composition (e and g) and percentage contribution of each modification (f and h) from wild-type and Idua^−/− liver (e and f) and brain (g and h). Results are an average from three independent animals with two separate heparinase digests per animal. n = 3. The bars represent mean ± S.D. p values are quoted using a Student's t test and assuming two-tailed distribution and equal variance. NAc, N-acetylated glucosamine; NS, N-sulfated glucosamine; 2S, 2-O-sulfate group; 6S, 6-O-sulfate group.