FIGURE 7.

Activity of the domain-minimized H2 relaxin analogues on the RXFP2 receptor. A, competition binding results of the A- and B-chain-truncated minimized analogues, H2-(B7–24) and H2-(A4–24)(B7–24) compared with INSL3 and H2 relaxin in HEK-293T cells stably expressing the RXFP2 receptor using Eu³⁺-labeled H2 relaxin as the competitive ligand. Data are expressed as percentage of specific binding and are pooled data from at least three experiments performed in triplicate. B, cAMP activity of the A- and B- chain-truncated minimized analogues, H2-(B7–24) and H2-(A4–24)(B7–24) compared with H2 relaxin in HEK-293T cells expressing the RXFP2 receptor using a pCRE-β-galactosidase reporter gene system. Data are expressed as percentage of maximum H2 relaxin-stimulated cAMP response and are pooled data from at least three experiments performed in triplicate.