FIGURE 6.

AUP1 is involved in the accumulation of lipid droplets. A, HeLa cells were transduced with the shRNA constructs used in Fig. 1. Three days post-transduction, cells were left untreated or incubated with 0.4 mm oleic acid for 16 h. Lipid droplets were stained with BODIPY 493/503, and the amount of cellular fluorescence was determined by flow cytometry. Error bars represent standard deviation from three independent experiments. * indicates p < 0.05 by Student's t test. B, HeLa cells were transfected with empty vector or HA-AUP1 (WT or mutants, as indicated). One day post-transfection, cells were left untreated or incubated with 0.4 mm oleic acid for 16 h. Lipid droplets were stained with BODIPY 493/503, and the amount of cellular fluorescence was determined by flow cytometry. Error bars represent standard deviation from three independent experiments. * indicates p < 0.05 by Student's t test.