FIGURE 6.

Acyl-CoA and LPA specificity of recombinant human AGPAT1 and AGPAT2 expressed in AD293 cells. Specificity of human recombinant AGPAT1 (A) and AGPAT2 (C) for acyl-CoA donors was determined using sn-1-oleoyl-lysophosphatidic acid as an acceptor and various short, medium, and long chain fatty acyl-CoA as donors. The enzymatic activity is also compared with C18:1 = 100%, which is shown across the graph as a broken line. Specificity of human recombinant AGPAT1 (B) and AGPAT2 (D) for various species of sn-1-lysophosphatidic acid acceptors was determined using radioactive C18:1 as donor. Activities are expressed as product [³H]PA formed (nmol per min per mg protein). Shown is the 100 and 50% enzymatic activity when compared with C18:1 = 100%. All enzymatic activities were determined in two independent experiments in triplicate. Shown are the means from individual experiments, and each bar represents the mean of two experiments.