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. 2011 Sep 9;286(43):37732–37740. doi: 10.1074/jbc.M111.242826

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — ZIC2 binding to TCF4. Cell lysates of 293T cells transfected with ZIC2-FLAG- and β-catenin-expressing plasmids together with TCF4-expressing vector (A) or without TCF4 (B) were subjected to immunoprecipitation (IP) using anti-FLAG antibody. C, schematic representation of LEFΔN-β-cateninΔN53 fusion protein. The graph shows the TOPflash reporter activity of 293T cells after transfection with the expression plasmids as indicated. D, Western blot showing the levels of the indicated proteins after FLAG-tagged ZIC2 overexpression in 293T cells.