Skip to main content
. 2011 Aug 31;286(43):37758–37767. doi: 10.1074/jbc.M111.248039

FIGURE 1.

FIGURE 1.

Cilp2 is expressed in joint cartilage but not in growth plate cartilage. A, RT-PCR analysis demonstrates that Cilp2 is expressed in articular cartilage (AC) but not in proliferative (PR), prehypertrophic (PH), and hypertrophic (H) growth plate cartilage. Articular cartilage, and proliferative, prehypertrophic, and hypertrophic growth plate cartilage was microdissected from 2-week-old wild type mice. The RNA was isolated, linearly amplified, reverse transcribed, and subjected to 30 rounds of PCR. Gapdh was used as a positive control. B, an antisense probe was used to determine the distribution of Cilp2 mRNA in joint and growth plate cartilage. Darkfield images show that Cilp2 was expressed in at the surface and in the intermediate zone of joint cartilage and throughout meniscal cartilage. Cilp2 mRNA was not detected in growth plate cartilage. No hybridization with the sense probe was detected. MC, meniscal cartilage; R, resting zone; PR, proliferative zone; PH, prehypertrophic zone; H, hypertrophic zone; TB, trabecular bone. Scale bar, 100 μm.