FIGURE 3.

CRMP2 knockdown is neuroprotective. E18–19 neurons grown for 2 DIV were incubated with 1.45 × 10¹⁰ lentiviral particles for 5 days and then stimulated with 200 μm glutamate and 100 μm d-serine for 30 min. Cell viability was measured 24 h later with the MTS assay, with all values normalized to no stimulation control. Knockdown of CRMP2 significantly increased cell viability in the face of an excitotoxic challenge (*, p < 0.05 versus control shRNA; Student's t test). Neurons infected with CRMP2 shRNA and then additionally incubated with TAT-CBD3 peptide for 10 min prior to stimulation showed compete resistance to cell death (*, p < 0.05 versus control shRNA; Student's t test). Error bars, S.E.