FIGURE 7.

TAT-CBD3 does not alter surface NR2B expression. A, representative immunoblots with the indicated antibodies of total lysates (T), unbiotinylated fractions (U), and biotinylated fractions (B) prepared from neurons exposed to TAT-Control or TAT-CBD3 (10 μm each). B, summary of biotinylated (surface) NR2B protein, calculated by densitometry from two experiments. Values were normalized to time-matched TAT-Control conditions (n = 6–11 each). Neurons were left untreated (control) or stimulated for 30 min with Glu/Ser followed by biotinylation 0.5–2 h later. Levels of surface NR2B were not different in any of the conditions tested (p > 0.05; Student's t test).