FIGURE 9.
LPS-binding activities of wild-type and mutant Hb subunits. ELISA was performed for quantifying the LPS-binding activity of the mutant Hb subunits: Hb α (A), and Hb β (B). 96-Well MaxisorpTM microtiter plates were coated with S. minnesota LPS. The unbound sites were blocked with 2% BSA, and increasing concentrations of the recombinant proteins were added to each well. Anti-Hb antibody was added followed by horseradish peroxidase-linked secondary antibody. The peroxidase enzyme activity was determined at 405 nm. Results are the means ± S.D. of three independent experiments done in triplicate.