FIGURE 4.
In vitro activity of the H. pylori FucT on the in vivo generated precursor glycoprotein. Fucosylation was determined after in vitro incubation of purified AcrA glycoproteins containing the H. influenzae-derived tetrasaccharide with extracts of E. coli cells expressing the H. pylori fucosyltransferases and radiolabeled GDP-fucose. The fucosyltransferases used were H. pylori 11639 FucT full-length (11639FL) and truncated (11639TR) versions or as negative control the empty vector pEXT20. The counts were collected from experiments conducted in triplicates.