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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Cancer Cell. 2011 Oct 18;20(4):487–499. doi: 10.1016/j.ccr.2011.09.004

Figure 4. SIRT2 Interacts with APC/C and Deacetylates CDH1 and CDC20.

Figure 4

(A) Mass spectrometry analysis of Flag-SIRT2-interactingproteins after transfection of Flag-SIRT2 into HeLa cells. Lysates were immunoprecipitated with anti-Flag-agarose beads and eluted using Flag peptide, resolved by SDS-PAGE gel, and subjected to mass spectrometry analysis.

(B) Immunoblots of Flag-SIRT2 interacting proteins.

(C–F) Reciprocal co-immunoprecipitation of endogenous SIRT2 and CDH1 (C,D) or CDC20 (E,F) from immortalized SIRT2+/+ and SIRT2−/− MEF cells. After synchronization using serum starvation for 72 hr, cells were released into normal media for 16 hr and harvested for co-immunoprecipitation. The lysates were immunoprecipitated with anti-SIRT2, -CDH1, or CDC20 antibodies, respectively, and IPed samples were analyzed by immunoblotting with anti-SIRT2, -CDH1, or –CDC20, respectively.

(G,H) In vivo deacetylation analysis of endogenous CDH1 (G) and –CDC20 (H) from liver of SIRT2 WT and KO mice 48 hours after partial hepatectomy.

(See also Figure S4)