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. Author manuscript; available in PMC: 2011 Oct 24.
Published in final edited form as: Hum Mol Genet. 2010 Jan 7;19(6):1119–1128. doi: 10.1093/hmg/ddp584

Figure 3.

Figure 3

Reduced basal NOS function in Col4a1+/Raw. (A) Contraction obtained by administration of L-NAME as measure of amount of NO in 11-15 month animals expressed as percentage of contraction obtained with KCl. (n= 14 WT, n=11 Col4a1+/Raw, t-test p <0.05). (B) qRT-PCR for PRKG1 (cGMP dependant protein kinase I). Expressed as relative units to 18S RNA levels. (n=6) (C). eNOS mRNA levels in 12 month old WT (white) and Col4a1+/Raw (black) (n=6) 3 animals as determined by qRT-PCR relative to 18S RNA levels (p = 0.17 t-test). (D) eNOS, and sGC protein expression levels in aortae of 11-15 month old animals. Predominant band of coomassie gel is given as loading control (entire gel is provided in Supplemental Fig. 3). Error bars indicate standard error of measurement. * p< 0.05 t-test.