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. Author manuscript; available in PMC: 2011 Oct 24.
Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8978–8987. doi: 10.1021/bi060440o

Figure 10. Reaction of GRx1 and adduct with DTT.

Figure 10

GRx1 was pretreated with sporidesmin, followed by incubation in the presence of 35 mM DTT for 1 h at room temperature, then subjected to mass spectral analysis. MALDI ions at m/z 11,613 +/− 2 and 443 were consistent with release of GRx1 and sporidesmin, respectively, each less a sulfur atom, as would be expected if two sulfurs had been eliminated from the adduct. Ions consistent with the presence of unreacted GRx1 (11,645) and SP-GRx1 adduct minus 64 Da (12,054), reflecting possible loss of two sulfur atoms, are also evident.