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. Author manuscript; available in PMC: 2011 Oct 24.
Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8978–8987. doi: 10.1021/bi060440o

Figure 9. Alkylation of GRx1 cysteines with iodoacetamide (IAM).

Figure 9

MALDI spectra of GRx1 reacted with iodoacetamide (A), and GRx1 first reacted with sporidesmin, then with iodoacetamide (B). IAM-treated GRx1 contained a major ion m/z 11,930 +/− 3 consistent with alkylation of all five cysteines. MALDI analysis of the products of reaction of GRx1 with sporidesmin, then treated with IAM, only showed major ions corresponding to the SP-GRx1 adduct (m/z 12054) and GRx1 modified by carboxamidomethylation of a single cysteine (calculated m/z 11,702).