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EPR spectra of BtuB G170R1 reconstituted into POPC and DMPC bilayers, and spectra from 4 mutations surrounding G170R1, reconstituted into POPC bilayers, where single alanine mutations were made to the nearest neighboring residues. These spectra include those from mutations to the i±2 residues (Y172, L168), the hydrogen-bonded (V154) and non-hydrogen bonded (L202) neighbors. All spectra are 100 Gauss scans.
