Table 1.
XmY | XmXp | Xm | Xp | Total | |
---|---|---|---|---|---|
Dnmt3l+/− | 59 | 61 | 0 | 0 | 120 |
Dnmt3l+/+ | 57 | 59 | 2 | 1 | 119 |
Total | 116 | 120 | 2 | 1 | 239 |
Genomic DNA from F1 hybrid offspring of C57BL/6 Dnmt3l+/− sires crossed with FVB/N dams was prepared at 10.5 d.p.c. The presence or absence of the Y chromosome was determined by Sry PCR, and the Dnmt3l genotype was determined by PCR (see Methods). Two polymorphic microsatellite markers (DXMit117 and DXMit1) were used to determine parental origin of the X chromosomes. The maternal (Xm) and paternal (Xp) X chromosomes are indicated.