Figure 6.
Cisplatin increases inflammation in rat cochlea. (a) Rats were treated with scramble or STAT1 siRNA by trans-tympanic injections followed by vehicle or cisplatin (11 mg/kg, i.p.) 48 h later. The rats were killed 72 h following the administration of vehicle or cisplatin. The cochleae were excised and processed for immunohistochemistry. Mid-modiolar sections of the cochlea were co-labeled with CD14 and TNF-α antibodies, followed by fluorescein- (green) or TRITC (red)-labeled secondary antibodies. Cisplatin increased CD14 and TNF-α immunoreactivity in cochleae treated with scramble siRNA. However, the increases in immunolabeling were attenuated in cochlea pretreated with STAT1 siRNA. The merged panels (yellow) indicate colocalization of CD14 and TNF-α. The scale bars (right lower panel) indicate 50 μm. (b) Magnified view of the OHCs presented in panel a. The arrows indicate three rows of OHCs. The scale bar indicates 10 μm