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. 2011 Jul 21;2(7):e181. doi: 10.1038/cddis.2011.62

Figure 5.

Figure 5

TFP augments the activation of both the extrinsic and the intrinsic apoptotic pathways after DNA damage. U1810 cells were exposed to DNA-damaging drugs (2.5 μg/ml bleomycin, 20 μM cisplatin) alone or in combination with TFP (10 μM). For immunoblotting and FLICA assay, samples were collected 48 h post DNA-damaging treatment. (a) TFP augmented caspase-3 activation after bleomycin treatment (left); enhanced caspase-3 activation was detected predominantly in cells containing 4n DNA content (right). (b) TFP potentiated caspase-3 activation after cisplatin treatment. (c) TFP co-treatment resulted in increased cleavage of PARP. (d) TFP co-treatment resulted in increased cleavage of caspase-8. (e) TFP co-treatment resulted in increased cleavage of caspase-9. (f) TFP-co-treated cells contain increased levels of catalytically active caspase-9. For (a, b and f), mean and S.D. were compiled from three independent experiments performed in duplicates (*P<0.05, TFP co-treatment versus single-agent bleo/cis treatment). For (c, d and e), the numbers at the top indicate the normalized intensity of the bands. One representative experiment is shown (n=3)