Figure 4.

Investigation of adhesion, spreading and migration of integrin-deficient hMSC on collagen I. (a) Quantitative adhesion assay demonstrated that α2- and α11-shRNA-transduced hMSC have reduced attachment to collagen I in comparison with α1- and con-shRNA hMSC. Data consist of two independent infections; experiments were performed in triplicate. (b) Time lapse analysis of cells spreading on collagen I showed significant delay of hMSC deficient for α2 or α11 subunits. (c) Shown is a panel of images representative of the migration distance taken by a single control or shRNA-transduced hMSC. Cell tracks are indicated with black arrows. Quantification of migration distance (d) and velocity (e) revealed significantly lower motility of α2- and α11-shRNA hMSC. Time-lapse analysis was performed after two independent infections. Data in b, d and e are representative of three independent movies as a total of 30 cells per type were evaluated (n=3; ^**P<0.01; ^***P<0.001; ANOVA tested versus con-shRNA)