Figure 5.

Cell regression of hMSC after integrin KD and investigation of IFN pathway-related genes. (a) Subsequent to the antibiotic selection, cells were pictured every 24 h for a period of 14 days. Shown are representative images at day 0 and 14. (b) hMSC deficient for α2 or α11 integrin decrease in number during cultivation in vitro. In contrast, α1-shRNA and con-shRNA hMSC were able to expand. In the text inset, the total cell gain or cell loss between day 0 and 14 is shown in percentage. The experiment was performed in duplicates with two donors as the total cell number in 1.1 cm² area was automatically counted by ImageJ software. (c) RT-PCR was performed for OAS1, OAS2, retinoic acid-inducible gene I (RIG-I) and IFN-induced protein with tetratricopeptide repeats 1 (IFIT1), genes involved in IFN-related cell death. The PCR results showed that none of the tested genes were upregulated after viral transduction of shRNA. Shown is a representative experiment of three independent infections. Positive control: hMSC treated with 2 × 10³U/ml IFNβ for 72 h