Figure 6.

Investigation of the mitochondrial integrity of integrin-deficient hMSC. Representative microphotographs of hMSC cultivated on collagen I and stained with JC-1. JC-1 dye aggregates and emits red color in cells with intact mitochondria such as non-treated hMSC (a), con- (c) and α1-shRNA hMSC (d) whereas apoptotic cells are labeled in green as in the positive control – hMSC treated with 1 μg/ml FAS antibody (b). hMSC with α2 (e) or α11 (f) KD are labeled in green indicating that their mitochondria are permeabilized. Cytochemistry was performed twice independently with two donors