Figure 4.

EMT induction upregulates ABC transporter expression. Graphs represent relative mRNA expression of EMT markers (a) and ABC transporters (b) after 1-week treatment of MCF7 cells with TGFβ, n=4. Error bars denote ±S.E.M. ^*P<0.05; ^**P<0.01. Graphs represent relative mRNA expression of EMT markers (c) and ABC transporters (d) in MCF7 cells transiently overexpressing mTwist, n=4. Error bars denote ±S.E.M. ^*P<0.05; ^**P<0.01. (e) Phase-contrast images of parent HMLE cells and HMLE cells stably overexpressing mTwist (HMLE-mTwist); scale bar=100 μm. (f) Graph represents distance migrated by cells in wound migration assay calculated as a difference of distance between the two edges of the wounds at 0 and 24 h measured in μm using the ProgRes capture software, n=4. Error bars denote ±S.E.M. ^**P<0.01. (g) Graph represents cell invasion in arbitrary units (AU) calculated as a ratio of cells invaded through Matrigel to the total number of cells plated, n=3. Error bars denote ±S.E.M. ^**P<0.01. Graphs represent relative mRNA expression of EMT markers (h) and ABC transporters (i) in HMLE cells stably overexpressing mTwist, n=3. Error bars denote ±S.E.M. ^*P<0.05. (j) Dose–response curves of parent HMLE cells versus HMLE cells stably expressing mTwist/FOXC2/Snail treated with doxorubicin for 48 h. The dotted lines represent the IC₅₀ values. (k) Graph represents relative mRNA expression of 16 ABC transporters in HMLE-mTwist cells after 1 week of Dox treatment, n=3. Error bars denote ±S.E.M. ^*P<0.05; ^**P<0.01; ^***P<0.001