Figure 5.

Reversal of EMT leads to increased drug sensitivity. (a) Photomicrographs of MDAMB231 cells transfected with control, Twist, or Zeb1 siRNA in the presence and absence of Dox treatment for 72 h. Scale bar=100 μm. Gel pictures depict RT-PCR analysis of EMT markers (b) and ABC transporters (c) in MDAMB231 cells transfected with control, Twist, or Zeb1 siRNA in the presence and absence of Dox treatment for 72 h. (d) Dose–response curves of MDAMB231 cells transfected with control, Twist, or Zeb1 siRNA, and subsequently treated with varying concentrations of Dox for 48 h. The dotted lines represent the IC₅₀ values. Error bars denote ±S.E.M. ^*P<0.05; ^**P<0.01. (e) Graph represents percentage cell invasion through Matrigel in Boyden chamber assay for MDAMB231 cells transfected with control or Twist siRNA, and subsequently treated with Dox for 72 h, n=3. Error bars denote ±S.E.M. ^*P<0.05; ^***P<0.001