Table 1.
Phenol-soluble proteins identified from 2-D (IEF-SDS-PAGE) gels of the acidic (pH 3-5.4; spot nos. A2- 40 in figure 2) and basic (pH 5.4-10; spot nos. B4-37 in figure 2) region proteins from C. comosa rhizomes, as analyzed by LC/MS/MS.
| Spot | Uniport ID | Protein name | Organism | Sequence coverage (%) | Peptides matched | Theoretical | Function | |
|---|---|---|---|---|---|---|---|---|
| MW (Da) | pI | |||||||
| A2 | Q40687 | Guanine nucleotide-binding protein subunit beta | Oryza sativa | 3 | 1 | 41,726 | 7.13 | Protein signaling |
| A3 | Q9FNA6 | Genomic DNA, chromosome 5, P1 clone | Arabidopsis thaliana | 6 | 1 | 60,231 | 5.56 | Unknown |
| A5 | P32033 | Protein ycf2 | Cuscuta reflexa | 7 | 1 | 234,393 | 9.25 | ATP binding |
| A8 | O64637 | Cytochrome P450 76C2 | Arabidopsis thaliana | 3 | 2 | 57,221 | 6.50 | Oxidoreductase |
| A7 | Q9MAZ0 | Nonclathrin coat protein | Zea mays | 6 | 1 | 19,928 | 4.81 | Protein transport |
| A9 | P30182 | DNA topoisomerase 2 | Arabidopsis thaliana | 2 | 2 | 164,005 | 7.25 | ATP binding |
| A11 | Q6ZBQ5 | Hypothetical protein | Oryza sativa | 7 | 1 | 15,105 | 11.6 | Unknown |
| A12 | Q9ZPH2 | Monothiol glutaredoxin-S17 | Arabidopsis thaliana | 3 | 1 | 53,082 | 5.01 | Electron carrier |
| A13 | P27898 | Myb-related protein P | Zea mays | 2 | 1 | 43,729 | 10.1 | Transcription |
| A16 | A9SAC0 | Predicted protein | Physcomitrella patens subsp patens | 4 | 1 | 17,421. | 5.28 | Unknown |
| A17 | Q9LDQ8 | Similarity to proton pump interactor | Arabidopsis thaliana | 1 | 1 | 58,552 | 5.66 | Unknown |
| A18 | Q2QM00 | Type IIB DNA topoisomerase family protein | Oryza sativa | 1 | 1 | 55,342 | 5.62 | DNA/ATP binding |
| A19 | Q9LVP9 | Vesicle transport v-SNARE 13 | Arabidopsis thaliana | 4 | 1 | 25,026 | 9.41 | Protein transport |
| A20 | Q5I2R0 | minus agglutinin (SAD1) | Chlamydomonas incerta | 1 | 2 | 404,525 | 6.08 | Defense |
| A25 | Q9GEZ3 | NADH dehydrogenase subunit F | Gymnosteris parvula | 2 | 1 | 37,892 | 9.74 | Oxidoreductase |
| A27 | Q6V8L5 | Lectin | Typhonium divaricatum | 12 | 4 | 20,250 | 9.17 | Defense |
| A31 | Q6RHR1 | Basic beta-1,3-glucanase | Capsicum annuum | 6 | 1 | 17,521 | 11.3 | Metabolism |
| A28 | O49565 | Putative F-box protein At4g21240 | Arabidopsis thaliana | 2 | 1 | 48,377 | 5.83 | Unknown |
| A34 | Q41625 | Mannose-binding lectin precursor | Tulipa hybrid cultivar | 13 | 4 | 19,556 | 5.60 | Defense |
| A40 | Q0ZIZ4 | ATP-dependent Clp protease proteolytic subunit | Vitis vinifera | 5 | 1 | 22,060 | 4.75 | Proteolysis |
| B4 | P93262 | Phosphoglucomutase | Mesembryanthemum crystallinum | 2 | 1 | 63,446 | 5.87 | Metabolism |
| B5 | Q94CI8 | Glycine-rich protein | Solanum lycopersicum | 2 | 1 | 23,420 | 5.88 | Unknown |
| B6 | Q1PCD2 | glucose 6 phosphate isomerase | Solanum lycopersicum | 5 | 2 | 62,739 | 6.56 | Metabolism |
| B7 | Q8LKB0 | enolase | Musa acuminata | 21 | 2 | 15,864 | 7.83 | Metabolism |
| B8 | Q06H19 | UDP glucose pyrophosphorylase | Arachis hypogaea | 22 | 3 | 16,851 | 7.30 | Metabolism |
| B9 | Q8S9B8 | UGPase PC | Pyrus pyrifolia | 3 | 1 | 50,719 | 5.90 | Metabolism |
| B10 | B2X0E6 | glyceraldehyde 3 phosphate dehydrogenase | Mallotus nesophilus | 47 | 2 | 32,795 | 5.96 | Metabolism |
| B11 | Q5PY03 | glyceraldehyde 3 phosphate dehydrogenase | Musa acuminata | 2 | 1 | 35,974 | 6.20 | Metabolism |
| B12 | Q2XQF4 | glyceraldehyde 3 phosphate dehydrogenase | Elaeis guineensis | 8 | 2 | 32,135 | 7.42 | Metabolism |
| B13 | P34922 | glyceraldehyde 3 phosphate dehydrogenase | Pisum sativum | 9 | 3 | 36,586 | 6.63 | Metabolism |
| B14 | A5JEJ7 | glyceraldehyde 3 phosphate dehydrogenase | Zehneria keayana | 13 | 1 | 7,001 | 9.87 | Metabolism |
| B15 | P84733 | Putative cytochrome c oxidase subunit II PS17 | Pinus strobus | 50 | 1 | 33,265 | 7.42 | Unknown |
| B16 | O82450 | branched chain alpha keto acid decarboxylase | Arabidopsis thaliana | 2 | 1 | 38,709 | 6.27 | Oxidoreductase |
| B17 | Q5ILG5 | cysteine protease gp3a | Zingiber officinale | 4 | 4 | 52,062 | 6.17 | Peptidase |
| B18 | Q01H20 | Predicted ATPase (ISS) | Ostreococcus tauri | 1 | 1 | 57,490 | 5.84 | ATP binding |
| B19 | P25251 | cysteine protease COT44 | Brassica napus | 4 | 1 | 36,277 | 8.05 | Peptidase |
| B21 | Q9FE01 | L-ascorbate peroxidase 2 | Oryza sativa | 15 | 3 | 27,101 | 5.21 | Stress/Defense |
| B23 | Q41561 | Heat shock protein 16.9C | Triticum aestivum | 16 | 2 | 14,376 | 6.23 | Stress |
| B24 | O22373 | Superoxide dismutase [Cu-Zn] | Capsicum annuum | 10 | 2 | 15,279 | 5.13 | Stress/Defense |
| B33 | Q8LEA2 | Gibberellin 2-beta-dioxygenase 1 | Arabidopsis thaliana | 7 | 2 | 36,709 | 8.53 | Oxidoreductase |
| B34 | Q94A43 | BES1/BZR1 homolog protein 2 | Arabidopsis thaliana | 8 | 1 | 34,174 | 8.63 | Transcription |
| B37 | Q09023 | Endochitinase CH25 precursor | Brassica napus | 8 | 1 | 34,793 | 6.29 | Metabolism |